diff --git a/.evergreen/scripts/setup_tests.py b/.evergreen/scripts/setup_tests.py
index dbf87d28d5..ba42a98106 100644
--- a/.evergreen/scripts/setup_tests.py
+++ b/.evergreen/scripts/setup_tests.py
@@ -555,9 +555,6 @@ def handle_test_env() -> None:
     if opts.green_framework:
         framework = opts.green_framework or os.environ["GREEN_FRAMEWORK"]
         UV_ARGS.append(f"--group {framework}")
-        if framework == "gevent" and opts.test_min_deps:
-            # PYTHON-5729.  This can be removed when the min supported gevent is moved to 25.9.1.
-            UV_ARGS.append('--with "setuptools==81.0"')
 
     else:
         TEST_ARGS = f"-v --durations=5 {TEST_ARGS}"
diff --git a/pyproject.toml b/pyproject.toml
index e7cf46c8ec..2a217a636a 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -64,7 +64,7 @@ unasync = [
     "unasync",
 ]
 pip = ["pip>=20.2"]
-gevent = ["gevent>=21.12"]
+gevent = ["gevent>=25.9.1"]
 coverage = [
     "coverage[toml]>=6.4,<=7.15.0"
 ]
diff --git a/uv.lock b/uv.lock
index 71d10dafa6..f14976519f 100644
--- a/uv.lock
+++ b/uv.lock
@@ -1514,7 +1514,7 @@ provides-extras = ["aws", "docs", "encryption", "gssapi", "ocsp", "snappy", "tes
 coverage = [{ name = "coverage", extras = ["toml"], specifier = ">=6.4,<=7.15.0" }]
 dev = []
 fuzz = [{ name = "atheris", specifier = ">=2.3.0" }]
-gevent = [{ name = "gevent", specifier = ">=21.12" }]
+gevent = [{ name = "gevent", specifier = ">=25.9.1" }]
 lint = [{ name = "ruff", specifier = "==0.15.20" }]
 mockupdb = [{ name = "mockupdb", specifier = ">=1.8.1" }]
 perf = [{ name = "simplejson", specifier = ">=3.17.0" }]